Trypsin-gold nanoparticle conjugates: binding, enzymatic activity, and stability.
نویسندگان
چکیده
Nanomaterials may interact with biomolecules in various ways and change their bioactivities. Here, we report on how gold nanoparticles (AuNPs) affect a most important protease, trypsin. After simple mixing of the trypsin and AuNPs solution the average diameter is 20 nm under enzyme friendly conditions (pH 8.0); the bond between trypsin and AuNPs was detected by UV-vis spectroscopy. The further protease assay of trypsin, before and after mixing with the AuNPs solution, pointed out an improved performance in terms of enzyme activity and stability.
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ورودعنوان ژورنال:
- Preparative biochemistry & biotechnology
دوره 39 4 شماره
صفحات -
تاریخ انتشار 2009